Part:BBa_K4436666:Design

Reverse Primer for Lambda Red CsgA knockout

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Had to ensure lower GC content so the annealing temperature wasn't high enough to denature the DNA

Source

40 nucleotide of homology to the csgA sequence, 20 nucleotide of homology to the pKD3 sequence.